Intestinal Neutral Ceramidase Deficiency Triggers Regulatory T Cell Response via Gd3 to Protect the Host from Intestinal Inflammation.
Article
Xu, Zhishan, Lei, Chao, Sriwastva, Mukesh K et al. (2026). Intestinal Neutral Ceramidase Deficiency Triggers Regulatory T Cell Response via Gd3 to Protect the Host from Intestinal Inflammation.
. Advanced Science, 13(5), e12681. 10.1002/advs.202512681
Xu, Zhishan, Lei, Chao, Sriwastva, Mukesh K et al. (2026). Intestinal Neutral Ceramidase Deficiency Triggers Regulatory T Cell Response via Gd3 to Protect the Host from Intestinal Inflammation.
. Advanced Science, 13(5), e12681. 10.1002/advs.202512681
Sphingolipids play a crucial role in gut inflammation. Neutral ceramidase (NcDase) serves as a pivotal regulator of ceramide, the central intermediate in sphingolipid metabolism. The contribution of intestinal epithelial cells (IEC) NcDase to colitis is not well understood. Here, a protective mechanism by which IEC NcDase deficiency (Asah2ΔIEC) and its-related gangliosides prevent dextran sulfate sodium (DSS)-induced colitis in mice is described. Asah2ΔIEC mice display reduced susceptibility to DSS-induced colitis and increase regulatory T (Treg) cells compared to Asah2fl/fl littermates. Deletion of IEC NcDase induces the upregulation of sialyltransferase ST8SIA1 and promotes the sialic-acid-containing ganglioside GD3 production. Siglec-E is a sialic-acid-binding lectin expresses predominantly on myeloid cells. Mechanistically, it is identified that GD3 is a functional ligand for Siglec-E on macrophages and found that GD3/Siglec-E interaction induced a rapid metabolic rewiring of macrophages that involved the production of IL-33, which contributes to the generation of ST2+Foxp3+ Treg cells. Finally, deletion of ST8SIA1 or administration of dietary GD3 induces or reduces mucosal inflammation, respectively. This work defines a critical role for ganglioside GD3 in the induction of colonic Treg cells and identifies an activating pathway that follows engagement of Siglec-E.
