Dual-functional probe based on rhodamine for sequential Cu2+ and ATP detection in vivo.
Article
Jin, Xilang, Gao, Jingkai, Xie, Pu et al. (2018). Dual-functional probe based on rhodamine for sequential Cu2+ and ATP detection in vivo.
. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 204 657-664. 10.1016/j.saa.2018.06.094
Jin, Xilang, Gao, Jingkai, Xie, Pu et al. (2018). Dual-functional probe based on rhodamine for sequential Cu2+ and ATP detection in vivo.
. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 204 657-664. 10.1016/j.saa.2018.06.094
A rhodamine-based fluorescent probe for Cu2+ and ATP has been designed. The fluorescence intensity/absorbance was significantly enhanced upon the addition of Cu2+ owning to the opening of the spiro-ring of rhodamine, which quickly returned to the original level due to the reconstruction of the probe by the reacting with ATP. Cu2+/ATP-induced fluorescent intensity/aborbance changes showed a good linear relationship with the concentration of Cu2+/ATP in the range of 2-20 μM/0-10 μM with a detection limit of 0.1 μM/1.0 μM. The proposed method is simple in design and fast in operation, and is suitable for the reversible monitoring of Cu2+ and ATP in bioanalytical applications.
