Applications of digital polymerase chain reaction in environment and forensic chemistry
Book Chapter
Etim, S, Raha, D, Duncan, G et al. (2025). Applications of digital polymerase chain reaction in environment and forensic chemistry
. 495-510. 10.1016/B978-0-443-30128-5.00032-1
Etim, S, Raha, D, Duncan, G et al. (2025). Applications of digital polymerase chain reaction in environment and forensic chemistry
. 495-510. 10.1016/B978-0-443-30128-5.00032-1
Digital polymerase chain reaction (dPCR), a cutting-edge biomolecular technique that offers sensitive quantification of nucleic acids with high specificity. This chapter reviews recent applications of dPCR in environmental and forensic chemistry. In environmental DNA (eDNA) analysis, dPCR allows for precise identification and monitoring of microbial pollutants for surface water/disease detection, bacterial contamination of food, as well as detection of rare and endangered species. In forensic chemistry, dPCR aids in the detection of microbes, distinguishing different body fluids, and in quantifying mixed, aged, and degraded forensic samples. The robust and versatile nature of dPCR makes it an effective tool to solve complex analytical tasks within these fields, boosting development in environmental monitoring and forensic science.
