This chapter presents the use of substance detection canines from a forensic and security viewpoint. It provides the reader with a foundational framework of forensic principles that can be extended and applied to the use of canine teams as a forensic tool for odor detection purposes. It draws a special focus on key aspects of legal and security considerations to better educate the reader on opti-mal and efficient uses of canine teams within operational settings. Furthermore, it provides a basic introduction to the chemical aspects of odor analysis as it relates to the detection of canine performance. Lastly, it provides a synopsis of detection disciplines within substance detection canines of relevance to the forensic practice.