Soybean trypsin inhibitor ameliorates haemorrhagic shock induced gastric lesions in the rat Article

Couse, NF, Grace, PA, Zinner, MJ. (1989). Soybean trypsin inhibitor ameliorates haemorrhagic shock induced gastric lesions in the rat . 5(4), 263-268.

cited authors

  • Couse, NF; Grace, PA; Zinner, MJ

authors

abstract

  • The effect of soybean trypsin inhibitor on acute gastric mucosal lesions, produced by haemorrhagic shock and intra-gastric hydrochloric acid instillation, was studied in the pylorus-ligated rat. Five minutes following intra-gastric injection of 0.1 N HCl, and immediately prior to the onset of haemorrhagic shock, soybean trypsin inhibitor (STI) 15 mg/kg was given intravenously. Control rats received an equal volume of 0.9% saline. Rats were subjected to haemorrhagic shock for 20 minutes and then the shed blood was reinfused. Following a 20 minute recovery period, rats were sacrificed and their stomachs removed. The gastric specimens were opened and scored in a blinded fashion for mucosal ulceration. The percentage area ulceration in the control group was 13.7 ± 3.6 compared to 3.3 ± 1.1 in the STI group (p < 0.02). In the control group, transgastric potential difference (PD -ve mVolts) fell from 33.9 ± 0.9 to 6.6 ± 2.2 mV (p < 0.002) during shock. In the STI group, PD fell from 35.2 ± 1.7 to 20.3 ± 4.7 (p < 0.005). The fall in potential difference was significantly greater in the control group compared to the STI group (p < 0.02). In this model, STI administration results in significant protection of gastric mucosa during haemorrhagic shock, possibly by inhibiting generation of oxygen-free radicals.

publication date

  • January 1, 1989

start page

  • 263

end page

  • 268

volume

  • 5

issue

  • 4