Inflexinol inhibits colon cancer cell growth through inhibition of nuclear factor-κB activity via direct interaction with p50 Article

Jung, OB, Ju, HO, Bang, YH et al. (2009). Inflexinol inhibits colon cancer cell growth through inhibition of nuclear factor-κB activity via direct interaction with p50 . MOLECULAR CANCER THERAPEUTICS, 8(6), 1613-1624. 10.1158/1535-7163.MCT-08-0694

cited authors

  • Jung, OB; Ju, HO; Bang, YH; Dong, CM; Jeong, HS; Lee, S; Kim, S; Lee, H; Kim, KB; Sang, BH; Jin, TH

authors

abstract

  • Kaurane diterpene compounds have been known to be cytotoxic against several cancer cells through inhibition of nuclear factor-κB (NF-κB) activity. Here, we showed that inflexinol, a novel kaurane diterpene compound, inhibited the activity of NF-κB and its target gene expression as well as cancer cell growth through induction of apoptotic cell death in vitro and in vivo. These inhibitory effects on NF-κB activity and on cancer cell growth were suppressed by the reducing agents DTT and glutathione and were abrogated in the cells transfected with mutant p50 (C62S). Sol-gel biochip and surface plasmon resonance analysis showed that inflexinol binds to the p50 subunit of NF-κB. These results suggest that inflexinol inhibits colon cancer cell growth via induction of apoptotic cell death through inactivation of NF-κB by a direct modification of cysteine residue in the p50 subunit of NF-κB. Copyright © 2009 American Association for Cancer Research.

publication date

  • June 1, 2009

published in

Digital Object Identifier (DOI)

start page

  • 1613

end page

  • 1624

volume

  • 8

issue

  • 6