Quantification of Plasma 8-Isoprostane by High-Performance Liquid Chromatography with Tandem Mass Spectrometry in a Case-Control Study of Lung Cancer. Other Scholarly Work

Ma, Lin, Sun, Dongxiao, Xiu, Guangli et al. (2022). Quantification of Plasma 8-Isoprostane by High-Performance Liquid Chromatography with Tandem Mass Spectrometry in a Case-Control Study of Lung Cancer. . INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 19(19), 12488. 10.3390/ijerph191912488

cited authors

  • Ma, Lin; Sun, Dongxiao; Xiu, Guangli; Lazarus, Philip; Vachani, Anil; Penning, Trevor M; Whitehead, Alexander S; Muscat, Joshua E

authors

abstract

  • Aim

    8-iso-prostaglandin F2α is a biomarker of lipid peroxidation, and one of the most commonly used measures of oxidative stress. It is an established biomarker of lung cancer risk. It is commonly measured by enzyme-linked immunosorbent assay. Given its importance, we developed a stable isotope dilution UPLC-tandem mass spectrometric method for the rapid determination of 8-isoprostane in blood.

    Methods

    We tested the discriminatory capability of the method in 49 lung cancer patients, 55 benign lung nodule patients detected by chest X-ray, and 41 patients with chronic obstructive pulmonary disease (COPD) or asthma.

    Results

    Significant differences were found in mean 8-isoprostane levels between the three groups (p = 0.027), and post-hoc tests found higher levels in the lung cancer patients than in patients with benign nodules (p = 0.032) and COPD/asthma (p = 0.014). The receiving operating characteristic area under the curve (AUC) was 0.69 for differentiating the lung cancer group from the benign nodule group, and 0.7 for differentiating from the COPD/asthma group.

    Conclusions

    The UPLC-MS/MS-based method is an efficient analytical tool for measuring 8-isoprostane plasma concentrations. The results suggest exploring its utility as a marker for early lung cancer screening.

publication date

  • September 1, 2022

keywords

  • Asthma
  • Biomarkers
  • Case-Control Studies
  • Chromatography, High Pressure Liquid
  • Chromatography, Liquid
  • Dinoprost
  • Early Detection of Cancer
  • Humans
  • Isotopes
  • Lung Neoplasms
  • Oxidative Stress
  • Pulmonary Disease, Chronic Obstructive
  • Tandem Mass Spectrometry

Digital Object Identifier (DOI)

Medium

  • Electronic

start page

  • 12488

volume

  • 19

issue

  • 19