The antioxidant activity of the methanolic extract of powder of roots of Phaseolus trilobus was evaluated. Methanolic extract was evaluated for its reducing power, hydrogen peroxide scavenging activity and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity. High content of flavonoids in the roots may account for the antioxidant activity of the species. Study shows the potential of the methanolic extract of P. trilobus as a natural antioxidant.