Metal Oxide Nanoparticle-Embedded Chitosan Matrix Based Electrochemical Detection of DNA Hybridization
Article
Thakur, H, Kaur, N, Prabhakar, N. (2014). Metal Oxide Nanoparticle-Embedded Chitosan Matrix Based Electrochemical Detection of DNA Hybridization
. 4(4), 322-328. 10.1007/s12668-014-0154-5
Thakur, H, Kaur, N, Prabhakar, N. (2014). Metal Oxide Nanoparticle-Embedded Chitosan Matrix Based Electrochemical Detection of DNA Hybridization
. 4(4), 322-328. 10.1007/s12668-014-0154-5
A nanocomposite has been prepared by doping chitosan with iron oxide nanoparticles to immobilize the single-stranded DNA (ssDNA) for detection of DNA hybridization. Different characterization studies such as Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), UV–visible spectroscopy, and electrochemical studies have been done to confirm the synthesis of CHIT–Fe3O4 hybrid nanocomposite as well as immobilization of the DNA. Differential pulse voltammetry (DPV) of the bioelectrodes (DNA/CHIT/ITO and DNA/CHIT–Fe3O4/ITO) has been done to detect the complementary sequences using methylene blue (MB) as electroactive indicator. The DNA/CHIT–Fe3O4/ITO bioelectrode shows better detection limit (0.1 fM) over the DNA/CHIT/ITO genosensor (1.0 pM) within 60 s.
