Aptamer based voltammetric biosensor for the detection of Mycobacterium tuberculosis antigen MPT64 Article

Thakur, H, Kaur, N, Sabherwal, P et al. (2017). Aptamer based voltammetric biosensor for the detection of Mycobacterium tuberculosis antigen MPT64 . MICROCHIMICA ACTA, 184(7), 1915-1922. 10.1007/s00604-017-2174-7

cited authors

  • Thakur, H; Kaur, N; Sabherwal, P; Sareen, D; Prabhakar, N

authors

abstract

  • The authors report an aptaelectrode based on graphene modified iron-oxide chitosan hybrid (CHIT-IO-GR) nanocomposite film deposited on fluorine tin oxide (FTO) for the detection of the Mycobacterium tuberculosis specific antigen MPT64. The biotinylated DNA aptamer sequence specific to the MPT64 was immobilized onto the CHIT-IO-GR/FTO electrode by using streptavidin-biotin interactions. XRD, FT-IR, FE-SEM and electrochemical studies were applied to monitor the steps of the fabrication. The aptaelectrode, operated best at typical voltage of 0.44 V, exhibited a limit of detection (LOD) of 0.9 fg⋅mL−1 within 20 min. The biosensor retained about 80% of its initial activity after 10 uses. The potential application of the aptasensor was established by spike-in studies to obtain recoveries between 83 and 95%. [Figure not available: see fulltext.].

publication date

  • July 1, 2017

published in

Digital Object Identifier (DOI)

start page

  • 1915

end page

  • 1922

volume

  • 184

issue

  • 7