Hyper-production of pullulan from de-oiled rice bran by Aureobasidium pullulans in a stirred tank reactor and its characterization
Article
Singh, RS, Kaur, N, Pandey, A et al. (2020). Hyper-production of pullulan from de-oiled rice bran by Aureobasidium pullulans in a stirred tank reactor and its characterization
. 11 10.1016/j.biteb.2020.100494
Singh, RS, Kaur, N, Pandey, A et al. (2020). Hyper-production of pullulan from de-oiled rice bran by Aureobasidium pullulans in a stirred tank reactor and its characterization
. 11 10.1016/j.biteb.2020.100494
Statistical optimization of fermentation conditions for production of pullulan from de-oiled rice bran (DRB) by Aureobasidium pullulans in a laboratory scale stirred tank reactor (STR) was carried out using a central composite rotatable design. Optimal fermentation conditions involving 250 rpm agitation, 0.93 vvm aeration and 7.9 days fermentation time supported maximum pullulan yield (8.32 ± 0.02%, w/v). The corresponding biomass yield and sugar utilization were 0.990 ± 0.01 (A600/100) and 96.79 ± 0.03% (w/w). ANOVA confirmed the significance of generated model. Coefficient of determination value close to 1.0 for pullulan production (0.9919), biomass yield (0.9914) and sugar utilization (0.9975) verified the significance of the model. Non-significant “Lack-of-Fit” value for all response variables showed fitness of polynomial quadratic model. Structure of pullulan was confirmed by its spectral analysis. XRD of pullulan verified its amorphous nature and thermal analysis showed its melting range from 293.08 °C to 343.13 °C. SEM analysis shown smooth surface of pullulan.
