Isolation, characterization and cross-species amplification of new microsatellite markers for three opossum species of the Didelphidae family Article

Dias, IMG, Amato, G, Cunha, HM et al. (2009). Isolation, characterization and cross-species amplification of new microsatellite markers for three opossum species of the Didelphidae family . CONSERVATION GENETICS RESOURCES, 1(1), 405-410. 10.1007/s12686-009-9094-8

cited authors

  • Dias, IMG; Amato, G; Cunha, HM; DeSalle, R; Paglia, AP; Peterson, JK; Fonseca, CG

authors

abstract

  • The American opossums from the Didelphidae family (Order: Didelphimorphia) are on the IUCN Red List, and their threatened status varies from extinct to data deficient. Although microsatellites have already been developed for some species, only a small amount of population genetic analysis of the didelphid wild populations has been made using them. In this work, we developed new microsatellites and tested for cross-species amplification for one species from the subfamily Caluromyinae, Caluromys philander, and two from the subfamily Didelphinae, Didelphis aurita and Metachirus nudicaudatus, to determine the degree of gene flow and isolation of populations of those species in some fragments of the Atlantic Forest in Minas Gerais state, Brazil. Among all microsatellite loci amplified, 18 from C. philander, 9 from D. aurita, and 17 from M. nudicaudatus were polymorphic. Cross-species amplification of these polymorphic microsatellite loci were performed in these three species and in one additional related species Micoureus paraguayanus. Our data showed that microsatellites were not highly conserved among Didelphidae species. © Springer Science+Business Media B.V. 2009.

publication date

  • December 1, 2009

published in

Digital Object Identifier (DOI)

start page

  • 405

end page

  • 410

volume

  • 1

issue

  • 1