The effects of ω-3 polyunsatulated fatty acids on the recovery of cardiac function following cold preservation and reperfusion in hyperlipidemic rats
Article
Ku, K, Nosaka, S, Hashimoto, M et al. (1996). The effects of ω-3 polyunsatulated fatty acids on the recovery of cardiac function following cold preservation and reperfusion in hyperlipidemic rats
. 62(6), 735-742. 10.1097/00007890-199609270-00006
Ku, K, Nosaka, S, Hashimoto, M et al. (1996). The effects of ω-3 polyunsatulated fatty acids on the recovery of cardiac function following cold preservation and reperfusion in hyperlipidemic rats
. 62(6), 735-742. 10.1097/00007890-199609270-00006
We examined the effects of supplementation with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), major components of ω-3 polyunsatulated fatty acids (PUFAs), on basal cardiac function and recovery of cardiac function of 'donor hearts' from adult (30 week) rats following cold preservation and reperfusion (P/R). In groups 1, 2, 3, and 4, respectively, 30-week-old rats were fed a soybean oil diet, a high- cholesterol oil (HC) diet, an HC diet with EPA, or an HC diet with DHA for 5 weeks. After collecting blood to analyze plasma levels of fatty acids among each group, the heart was excised and perfused on a Langendorff apparatus. Following evaluation of each rat's cardiac function, each heart was stored in HTK solution for 8 hr at 4°C. The heart was then reperfused and the coronary perfusate was collected to evaluate enzyme that had leaked. After cardiac functional recovery was estimated, myocardial fatty acids were measured. EPA supplementation significantly increases the plasma and cardiac levels of EPA as well as the ratio of EPA to arachidonic acid (AA). EPA supplementation also led to improved recovery of cardiac function following P/R, compared with that of rats who received soybean oil, high-cholesterol oil, and DHA. DHA supplementation significantly increased the plasma and cardiac levels of DHA as well as the ratio of DHA to AA-however, the cardiac functional recovery was almost identical to that of the rats who received high- cholesterol oil and was higher only than that of the rats who received soybean oil. There were no significant differences in enzyme that had leaked and myocardial water content among each group. These results suggest that alterations in the myocardial phospholipid composition by EPA supplementation may be profoundly responsible for attenuating myocardial I/R injuries. In contrast, DHA supplementation may not exert a cardioprotective effect following cold P/R. DHA supplementation alone may not increase the myocardial level of EPA enough to cause a protective effect against P/R injury. EPA supplementation to hyperlipidemic patients may be clinically warranted for increasing the potential number of donors.
