Bacterial diversity in deep-sea sediments under influence of asphalt seep at the São Paulo Plateau. Article

Queiroz, Luciano Lopes, Bendia, Amanda Gonçalves, Duarte, Rubens Tadeu Delgado et al. (2020). Bacterial diversity in deep-sea sediments under influence of asphalt seep at the São Paulo Plateau. . ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 113(5), 707-717. 10.1007/s10482-020-01384-8

cited authors

  • Queiroz, Luciano Lopes; Bendia, Amanda Gonçalves; Duarte, Rubens Tadeu Delgado; das Graças, Diego Assis; da Costa da Silva, Artur Luiz; Nakayama, Cristina Rossi; Sumida, Paulo Yukio; Lima, Andre OS; Nagano, Yuriko; Fujikura, Katsunori; Kitazato, Hiroshi; Pellizari, Vivian Helena


  • Here we investigated the diversity of bacterial communities from deep-sea surface sediments under influence of asphalt seeps at the Sao Paulo Plateau using next-generation sequencing method. Sampling was performed at North São Paulo Plateau using the human occupied vehicle Shinkai 6500 and her support vessel Yokosuka. The microbial diversity was studied at two surficial sediment layers (0-1 and 1-4 cm) of five samples collected in cores in water depths ranging from 2456 to 2728 m. Bacterial communities were studied through sequencing of 16S rRNA gene on the Ion Torrent platform and clustered in operational taxonomic units. We observed high diversity of bacterial sediment communities as previously described by other studies. When we considered community composition, the most abundant classes were Alphaproteobacteria (27.7%), Acidimicrobiia (20%), Gammaproteobacteria (11.3%) and Deltaproteobacteria (6.6%). Most abundant OTUs at family level were from two uncultured bacteria from Actinomarinales (5.95%) and Kiloniellaceae (3.17%). The unexpected high abundance of Alphaproteobacteria and Acidimicrobiia in our deep-sea microbial communities may be related to the presence of asphalt seep at North São Paulo Plateau, since these bacterial classes contain bacteria that possess the capability of metabolizing hydrocarbon compounds.

publication date

  • May 1, 2020


  • Alphaproteobacteria
  • Bacteria
  • Biodiversity
  • DNA, Bacterial
  • Deltaproteobacteria
  • Gammaproteobacteria
  • Geologic Sediments
  • High-Throughput Nucleotide Sequencing
  • Hydrocarbons
  • Metagenome
  • Metagenomics
  • Microbiota
  • RNA, Ribosomal, 16S
  • Seawater
  • Water Microbiology

Digital Object Identifier (DOI)


  • Print-Electronic

start page

  • 707

end page

  • 717


  • 113


  • 5