Brown seaweed Padina gymnospora is a prominent natural wound-care product
Article
Baliano, AP, Pimentel, EF, Buzin, AR et al. (2016). Brown seaweed Padina gymnospora is a prominent natural wound-care product
. 26(6), 714-719. 10.1016/j.bjp.2016.07.003
Baliano, AP, Pimentel, EF, Buzin, AR et al. (2016). Brown seaweed Padina gymnospora is a prominent natural wound-care product
. 26(6), 714-719. 10.1016/j.bjp.2016.07.003
Seaweeds are related to anti-inflammatory, anti-bacterial and anti-noceptive effects. This work aimedto verify the potential of seaweed Padina gymnospora (Kützing) Sonder 1871 to improve wound healingin vitro. P. gymnospora was collected at a bethonic area in Espirito Santo. Methanolic extract of P. gym-nospora was obtained by percolation. To determine cytotoxicity, colorimetric MTT tests were performedagainst normal fibroblasts (L929), macrophages (RAW 264.7) and human ovarian carcinoma (OVCAR-3)cell lines using concentration range of 12–110 μg ml−1. To evaluate in vitro wound healing, monolayer offibroblasts L929 was seeded and artificial wounded. Cell proliferation was blocked by 5 μg ml−1Myto-mycin C. Nitric oxide inhibition was quantified with Raw 264.7 by Griess reaction. Minimal inhibitoryconcentration (MIC) against Staphylococcus aureus was determined. Eletrospray ionization with Fouriertransform ion cyclotron resonance mass spectrometry (ESI-FT-ICR MS) was applied to detail compositionof P. gymnospora methanolic extract. No cytotoxic effect in all cell lines was detected until the maximumconcentration of 110 μg ml−1. P. gymnospora promoted significantly migration at the concentration of25 μg ml−1(p < 0.05). A prominent inhibition of nitric oxide formation was achieved in a concentration of20 μg ml−1of methanolic extract of P. gymnospora (62.06 ± 1.20%). Antibacterial activity against S. aureuscould be demonstrated with MIC of 500 μg ml−1. ESI-FT-ICR MS analysis indicated eleven moleculesbetween then, linolenic, oleic and linoleic acid. P. gymnospora favored wound repair in vitro what couldbe related to its fatty acid composition. In addition, its antimicrobial effect, and NO inhibition activitycontribute for a new approach of P. gymnospora as a promise natural product for treatment of cutaneouswound.
