Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System Article

Li, Shenglan, Xue, Haipeng, Long, Bo et al. (2015). Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System . JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (99), 10.3791/52539

Open Access International Collaboration

cited authors

  • Li, Shenglan; Xue, Haipeng; Long, Bo; Sun, Li; Tai, Truong; Liu, Ying

sustainable development goals

authors

publication date

  • May 1, 2015

webpage

published in

keywords

  • CELLS
  • CRISPR
  • Cas9
  • DNA CLEAVAGE
  • Developmental Biology
  • ENDONUCLEASE
  • GENE
  • IMMUNITY
  • Issue 99
  • MICE
  • Multidisciplinary Sciences
  • NUCLEASES
  • ONE-STEP GENERATION
  • RNA
  • Science & Technology
  • Science & Technology - Other Topics
  • VECTOR
  • double nickase
  • gene targeting
  • genetic engineering
  • genome editing
  • homologous recombination
  • human induced pluripotent stem cells
  • reporter cell line

Digital Object Identifier (DOI)

publisher

  • JOURNAL OF VISUALIZED EXPERIMENTS

issue

  • 99