Role of myofibril-inducing RNA in cardiac TnT expression in developing Mexican axolotl Article

Sferrazza, GF, Zhang, C, Jia, P et al. (2007). Role of myofibril-inducing RNA in cardiac TnT expression in developing Mexican axolotl . BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 357(1), 32-37. 10.1016/j.bbrc.2007.03.064

cited authors

  • Sferrazza, GF; Zhang, C; Jia, P; Lemanski, SL; Athauda, G; Stassi, A; Halager, K; Maier, JA; Rueda-de-Leon, E; Gupta, A; Dube, S; Huang, X; Prentice, HM; Dube, DK; Lemanski, LF

authors

abstract

  • The Mexican axolotl, Ambystoma mexicanum, has been a useful animal model to study heart development and cardiac myofibrillogenesis. A naturally-occurring recessive mutant, gene ``c", for cardiac non-function in the Mexican axolotl causes a failure of myofibrillogenesis due to a lack of tropomyosin expression in homozygous mutant (c/c) embryonic hearts. Myofibril-inducing RNA (MIR) rescues mutant hearts in vitro by promoting tropomyosin expression and myofibril formation thereafter. We have studied the effect of MIR on the expression of various isoforms of cardiac troponin T (cTnT), a component of the thin filament that binds with tropomyosin. Four alternatively spliced cTnT isoforms have been characterized from developing axolotl heart. The expression of various cTnT isoforms in normal, mutant, and mutant hearts corrected with MIR, is evaluated by real-time RT-PCR using isoform specific primer pairs; MIR affects the total transcription as well as the splicing of the cTnT in axolotl heart. © 2007 Elsevier Inc. All rights reserved.

publication date

  • May 25, 2007

published in

Digital Object Identifier (DOI)

start page

  • 32

end page

  • 37

volume

  • 357

issue

  • 1