In vivo multimodal nonlinear optical imaging of mucosal tissue Article

Sun, J, Shilagard, T, Bell, B et al. (2004). In vivo multimodal nonlinear optical imaging of mucosal tissue . OPTICS EXPRESS, 12(11), 2478-2486. 10.1364/OPEX.12.002478

cited authors

  • Sun, J; Shilagard, T; Bell, B; Motamedi, M; Vargas, G

authors

abstract

  • We present a multimodal nonlinear imaging approach to elucidate microstructures and spectroscopic features of oral mucosa and submucosa in vivo. The hamster buccal pouch was imaged using 3-D high resolution multiphoton and second harmonic generation microscopy. The multimodal imaging approach enables colocalization and differentiation of prominent known spectroscopic and structural features such as keratin, epithelial cells, and submucosal collagen at various depths in tissue. Visualization of cellular morphology and epithelial thickness are in excellent agreement with histological observations. These results suggest that multimodal nonlinear optical microscopy can be an effective tool for studying the physiology and pathology of mucosal tissue. © 2004 Optical Society of America.

publication date

  • May 31, 2004

published in

Digital Object Identifier (DOI)

start page

  • 2478

end page

  • 2486

volume

  • 12

issue

  • 11