Validation of HPLC analysis method of a novel antihypertensive agent MS23 in rat plasma Article

Wang, T, Fox, LM, Wang, D. (2006). Validation of HPLC analysis method of a novel antihypertensive agent MS23 in rat plasma . JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 830(1), 13-17. 10.1016/j.jchromb.2005.10.009

cited authors

  • Wang, T; Fox, LM; Wang, D

authors

abstract

  • MS23 is a vasodilator with unique dual action pharmacological profile to inhibit type 4 PDE and antagonize L-type calcium channels. We validated an analytical protocol for MS23 in rat plasma using high performance liquid chromatography (HPLC). A C18 column and a phosphate/acetonitrite buffer were used for chromatographic separation. UV detection was performed at 307 nm. The calibration curve for MS23 was linear in the range from 50 to 10,000 ng/ml. The limit of quantification (LOQ) was 50 ng/ml. The results demonstrate that the method has linearity (R = 0.9989), specificity, and acceptable precision/accuracy. This method is simple, economic, and sufficient for in vivo pharmacokinetic studies on the compound. © 2005 Elsevier B.V. All rights reserved.

publication date

  • January 2, 2006

published in

Digital Object Identifier (DOI)

start page

  • 13

end page

  • 17

volume

  • 830

issue

  • 1