Hydrogen peroxide promotes the activation of preeclampsia peripheral T cells
Article
Lv, J, Zhang, X, Wang, C et al. (2018). Hydrogen peroxide promotes the activation of preeclampsia peripheral T cells
. 24(4), 203-209. 10.1177/1753425918767305
Lv, J, Zhang, X, Wang, C et al. (2018). Hydrogen peroxide promotes the activation of preeclampsia peripheral T cells
. 24(4), 203-209. 10.1177/1753425918767305
Preeclampsia (PE) is a pregnancy disorder with a high mortality rate. Patients with PE exhibit systemic high oxidative stress status and inflammatory immune activation. This study aims to define the role of H 2 O 2 in the activation of neutrophils and T lymphocytes in PE patients. CD3 + /HLA-DR + cells in blood from PE patients are remarkably increased compared with those of normal non-pregnancies or normal pregnancies, while the percentage of CD3 + /CD62L + cells is significantly reduced in PE patients compared to normal pregnancies. Furthermore, CD62L levels in granulocytes of periphery blood of PE patients are significantly higher than non-pregnancies, but significantly lower than normal pregnancies. To characterize the effects of intracellular reactive oxygen species (ROS) on T lymphocyte activation in PE patients, PBMCs from normal pregnancies were challenged with H 2 O 2 , and intracellular ROS levels in neutrophil granulocytes, as well as T cell surface marker levels, have been determined. We confirm that H 2 O 2 exposure increases intracellular ROS levels in neutrophil granulocytes, and increases the proportion of CD3 + /HLA-DR + cells, but does not alter the percentage of CD3 + /CD62L + cells in PBMCs. Our study has confirmed dysregulated CD3 + /HLA-DR + and CD3 + /CD62L + T lymphocytes in PE patient peripheral blood, and the dysregulative effects of H 2 O 2 on T lymphocyte activation, suggesting a novel mechanism of immune activation in PE.