Effects of Pancreas Cold Ischemia on Islet Function and Quality Article

Pileggi, A, Ribeiro, MM, Hogan, AR et al. (2009). Effects of Pancreas Cold Ischemia on Islet Function and Quality . TRANSPLANTATION PROCEEDINGS, 41(5), 1808-1809. 10.1016/j.transproceed.2009.03.075

cited authors

  • Pileggi, A; Ribeiro, MM; Hogan, AR; Molano, RD; Embury, JE; Ichii, H; Cobianchi, L; Fornoni, A; Ricordi, C; Pastori, RL

authors

abstract

  • We used a rat model of pancreas cold preservation to assess its effects on islets. Glands were surgically retrieved and stored in University of Wisconsin (UW) solution for 3 hours (Short) or 18 hours (Long) cold ischemia time (CIT). Islet yield was significantly lower in the Long-CIT than the Short-CIT group, as well as islet recovery after overnight culture (P < .01). Islet cell viability after isolation was significantly reduced in the Long-CIT group (P < .05). Reversal of diabetes following transplantation of suboptimal islet grafts occurred earlier in the Short-CIT group than the Long-CIT. All animals in the Short-CIT group and 80% in the Long-CIT group achieved euglycemia. Freshly isolated islets showed a significant increase of JNK and p38 (P < .05) phosphorylation in Long-CIT compared with Short-CIT. Histopathological assessment of the pancreas showed a significantly higher injury score. Proteomic analysis of pancreatic tissue led to identification of 5 proteins consistently differentially expressed between Short-CIT and Long-CIT. Better understanding of the molecular pathways involved in this phenomenon will be of assistance in defining targeted interventions to improve organ use in the clinical arena. © 2009 Elsevier Inc. All rights reserved.

publication date

  • June 1, 2009

published in

Digital Object Identifier (DOI)

start page

  • 1808

end page

  • 1809

volume

  • 41

issue

  • 5