Beneficial effects of ischemic preconditioning on pancreas cold preservation Article

Hogan, AR, Doni, M, Damaris Molano, R et al. (2012). Beneficial effects of ischemic preconditioning on pancreas cold preservation . CELL TRANSPLANTATION, 21(7), 1349-1360. 10.3727/096368911X623853

cited authors

  • Hogan, AR; Doni, M; Damaris Molano, R; Ribeiro, MM; Szeto, A; Cobianchi, L; Zahr-Akrawi, E; Molina, J; Fornoni, A; Mendez, AJ; Ricordi, C; Pastori, RL; Pileggi, A

authors

abstract

  • Ischemic preconditioning (IPC) confers tissue resistance to subsequent ischemia in several organs. The protective effects are obtained by applying short periods of warm ischemia followed by reperfusion prior to extended ischemic insults to the organs. In the present study, we evaluated whether IPC can reduce pancreatic tissue injury following cold ischemic preservation. Rat pancreata were exposed to IPC (10 min of warm ischemia followed by 10 min of reperfusion) prior to ~ 18 h of cold preservation before assessment of organ injury or islet isolation. Pancreas IPC improved islet yields (964 ± 336 vs. 711 ± 204 IEQ/pancreas; p = 0.004) and lowered islet loss after culture (33 ± 10% vs. 51 ± 14%; p = 0.0005). Islet potency in vivo was well preserved with diabetes reversal and improved glucose clearance. Pancreas IPC reduced levels of NADPH-dependent oxidase, a source of reactive oxygen species, in pancreas homogenates versus controls (78.4 ± 45.9 vs. 216.2 ± 53.8 RLU/μg; p = 0.002). Microarray genomic analysis of pancreata revealed upregulation of 81 genes and downregulation of 454 genes (greater than twofold change) when comparing IPCtreated glands to controls, respectively, and showing a decrease in markers of apoptosis and oxidative stress. Collectively, our study demonstrates beneficial effects of IPC of the pancreas prior to cold organ preservation and provides evidence of the key role of IPC-mediated modulation of oxidative stress pathways. The use of IPC of the pancreas may contribute to increasing the quality of donor pancreas for transplantation and to improving organ utilization. © 2012 Cognizant Comm. Corp.

publication date

  • November 12, 2012

published in

Digital Object Identifier (DOI)

start page

  • 1349

end page

  • 1360

volume

  • 21

issue

  • 7