Effect of cAMP on nucleoside metabolism II. Cell cycle dependence of thymidine transport Article

Roller, BA, Hirai, K, Defendi, V. (1974). Effect of cAMP on nucleoside metabolism II. Cell cycle dependence of thymidine transport . JOURNAL OF CELLULAR PHYSIOLOGY, 84(3), 333-342. 10.1002/jcp.1040840302

cited authors

  • Roller, BA; Hirai, K; Defendi, V

authors

abstract

  • We have shown previously that adenosine 3′5′ cyclic monophosphate (cAMP) increased the transport of thymidine into monkey cells (CV‐1) without affecting DNA synthesis (Roller et al., '74). Using techniques of cell synchronization, cell separation and DNA synthesis inhibitors in CV‐1 and other cell lines, we now report that thymidine transport at low thymidine concentrations increases during the S phase of the cell cycle and that initiation of DNA synthesis is necessary for thymidine transport, although continuation of DNA synthesis is not necessary. Cyclic AMP alters nucleoside transport by increasing the Vmax for the transport reaction, but has no effect on the Km. Cyclic AMP is found not to affect the time in the cell cycle when thymidine transport occurs or the quality of the transport reaction, and only affects the amount of thymidine transported. Copyright © 1974 Wiley‐Liss, Inc.

publication date

  • January 1, 1974

published in

Digital Object Identifier (DOI)

start page

  • 333

end page

  • 342

volume

  • 84

issue

  • 3