Effect of cAMP on nucleoside metabolism. I. Effect on thymidine transport and incorporation in monkey cells (CV‐1) Article

Roller, B, Hirai, K, Defendi, V. (1974). Effect of cAMP on nucleoside metabolism. I. Effect on thymidine transport and incorporation in monkey cells (CV‐1) . JOURNAL OF CELLULAR PHYSIOLOGY, 83(2), 163-176. 10.1002/jcp.1040830202

cited authors

  • Roller, B; Hirai, K; Defendi, V

authors

abstract

  • The effect of cAMP on a monkey kidney cell line (CV‐1) noninfected and infected by SV40 was studied. No effect was found on either growth rate or cell morphology when concentrations up to 1 mM of 3′5′ cAMP were used. However, cAMP was found to increase the incorporation of 3H‐thymidine into both cellular and viral DNA without a net increase in DNA synthesis. This increased incorporation was found to be related to an enhanced uptake of thymidine into the nucleotide pool which is reflected in an increase of phosphorylated nucleotides. This, coupled with a lack of effect of cAMP on endogenous deoxyribonucleotide production, produces an increased specific activity of the deoxyribonucleotide triphosphates, with a resultant increase in specific activity of DNA. Copyright © 1974 Wiley‐Liss, Inc.

publication date

  • January 1, 1974

published in

Digital Object Identifier (DOI)

start page

  • 163

end page

  • 176

volume

  • 83

issue

  • 2