Involvement of recF in 254 nm ultraviolet radiation resistance in deinococcus radiodurans and escherichia coli Article

Chang, X, Yang, L, Zhao, Q et al. (2010). Involvement of recF in 254 nm ultraviolet radiation resistance in deinococcus radiodurans and escherichia coli . CURRENT MICROBIOLOGY, 61(5), 458-464. 10.1007/s00284-010-9638-x

cited authors

  • Chang, X; Yang, L; Zhao, Q; Fu, W; Chen, H; Qiu, Z; Chen, JA; Hu, R; Shu, W

authors

abstract

  • recF is a critical gene involved in the RecFOR pathway of DNA repair in Deinococcus radiodurans (D. radiodurans). To investigate the role of recF in UV-C radiation resistance, we generated the recF-deficient D. radiodurans strain R1, expressed recF in Escherichia coli (E. coli) BL21 cells, and compared the ability of each to resist UV-C radiation by F 10, inactivation constant (IC), and extrapolation number (N). The mutation of recF in D. radiodurans R1 resulted in characteristic slow growth and dramatic sensitivity to UV-C irradiation. Transformation of recF into E. coli BL21 cells resulted in increased UV-C resistance compared to untransformed E. coli BL21 cells. These results suggested that recF is needed for the replication of D. radiodurans. Furthermore, as a part of the RecFOR pathway in D. radiodurans, disruption of recF could dramatically decrease the UV-C resistance of D. radiodurans and recF could increase the UV-C resistance of E. coli BL21 cells. © 2010 Springer Science+Business Media, LLC.

publication date

  • November 1, 2010

published in

Digital Object Identifier (DOI)

start page

  • 458

end page

  • 464

volume

  • 61

issue

  • 5