Studies on isolation, identification and degradation characteristics of DBP-degradation strain CQ0302 Article

Li, J, Shu, WQ, Chen, JA et al. (2005). Studies on isolation, identification and degradation characteristics of DBP-degradation strain CQ0302 . 25(1), 47-51.

cited authors

  • Li, J; Shu, WQ; Chen, JA; Qiu, ZQ

authors

abstract

  • A di-n-butyl phthalate (DBP) degradation strain CQ0302 was isolated from rubbish landfill soil. According the morphological characteristics, biophysical biochemical and serial analyses of 16S rDNA, the strain was identified preliminarily as Rhodococcus ruber. The optimal temperature and pH were 30.0-35.0°C and 7.0-8.0 respectively in utilization of DBP by the strain for growing. The degradation dynamic equation was In C = -0.02735t + A when DBP concentration was lower than 1500 mg/L, with half life of 25.34 h. The results of strain wholecell protein SDS-polyacrylamid electrophoresis showed that there were very obvious differences of the total cell protein composition of the strain before and after DBP inducing. The activities of relative enzyme in the degradation process were determined showing that the aromatizing cracking was from the catalysis of catechol 1,2-dioxygenase.

publication date

  • February 1, 2005

start page

  • 47

end page

  • 51

volume

  • 25

issue

  • 1