Cloning expression and UVC resistant function of RecR and SSB proteins from Deinococcus radiodurans
Article
Chang, X, Yang, L, Fu, W et al. (2010). Cloning expression and UVC resistant function of RecR and SSB proteins from Deinococcus radiodurans
. 16(3), 328-331. 10.3724/SP.J.1145.2010.00328
Chang, X, Yang, L, Fu, W et al. (2010). Cloning expression and UVC resistant function of RecR and SSB proteins from Deinococcus radiodurans
. 16(3), 328-331. 10.3724/SP.J.1145.2010.00328
To construct the expression recombinants of RecR and SSB from Deinococcus radiodurans, express these target proteins in Escherichia coli BL21 (DE3) and analyze UVC resistant abilityies of RecR and SSB in E. coli, recR and ssb genes were amplified by PCR from genomic DNA of D. radiodurans and inserted into expression plasmid vector pET32a(+) to construct pET32a (+)-recR and pET32a(+)-ssb. The recombinant plasmids were then transformed into E. coli BL21 (DE3) and the recombinant proteins were expressed by induction with isopropyl-β-D-thiogalactopyranoside (IPTG) and analyzed with SDS-PAGE. Compared to the survival rate of bacteria in each group before and after UVC irradiation, the recombinant plasmids pET32a(+)-recR and pET32a(+)-ssb were obtained and these recombinant proteins could be highly expressed in E. coli BL21 (DE3). The results indicated that RecR and SSB from D. radiodurans could be expressed in E. coli BL21 successfully and could increase UVC resistance of E. coli. This study provides basic data for further studies on and future applications of the recombinants RecR and SSB.
