Evaluation of ovotoxicity in female mice caused by organic extracts in tap water from Jialing River in Chongqing, China Article

Xu, C, Zhang, JJ, Chen, JA et al. (2010). Evaluation of ovotoxicity in female mice caused by organic extracts in tap water from Jialing River in Chongqing, China . Birth Defects Research Part B: Developmental and Reproductive Toxicology, 89(1), 26-33. 10.1002/bdrb.20219

cited authors

  • Xu, C; Zhang, JJ; Chen, JA; Cao, B; Shu, WQ; Cao, J

abstract

  • BACKGROUND: Recently, toxic effects of widespread organic pollutants have received much attention due to the hazards they pose to female reproductive health. The aim of the present study was to determine the female reproductive toxicity of organic extracts (OE) in tap water from the Jialing River in Chongqing, China. METHODS: In our experiment, Kunming female mice that exhibited normal estrous cycles were randomly divided into 4 groups, which included a control group (OE 0 L/kg bw) as well as low- (OE 12.5 L/kg bw/day), mid- (OE 25 L/kg bw/day), and high-dose (OE 50 L/kg bw/day) groups. Mice were continually administered intraperitoneal injections of OE at different doses for 5 consecutive days. On the 15th and 30th day after treatments, half of the mice were sacrificed separately. RESULTS: The results showed that OE decreased relative ovary weights and prolonged the duration of estrous cycle with concomitant increase in estrous phase. There was a significant decrease in the number of corpora lutea of OE-treated mice, but no significant differences were found in healthy and atretic follicle populations compared to control. Ultrastructure observation regarding granulosa cells of the ovary revealed that OE treatment caused mitochondrial swelling together with endoplasmic reticulum expansion. CONCLUSIONS: All these data indicate that OE could exert adverse effects on the development of ovary and also a slight suppressive effect on reproductive functions. © 2009 Wiley-Liss, Inc.

authors

publication date

  • February 1, 2010

published in

Digital Object Identifier (DOI)

start page

  • 26

end page

  • 33

volume

  • 89

issue

  • 1