Antibiotic resistance and plasmid profile of thermotolerant coliform bacteria isolated from aquatic environment
Article
Liu, X, Shu, W, Qiu, Z et al. (2006). Antibiotic resistance and plasmid profile of thermotolerant coliform bacteria isolated from aquatic environment
. 12(1), 118-121.
Liu, X, Shu, W, Qiu, Z et al. (2006). Antibiotic resistance and plasmid profile of thermotolerant coliform bacteria isolated from aquatic environment
. 12(1), 118-121.
A total of 162 strains were isolated by membrane filter method and m-FC agar from aquatic environment, and identified by API system. The resistance to 10 kinds of most frequently used antibiotics in human and animals was analyzed by Kirby-Bauer method. The results showed that E. coli (96.3%) was the predominant strains of the all isolates. All isolates were resistant to three or more antibiotics except three strains isolated from spring, and the incidence of multiple antibiotic resistance (MAR) was 98.1%. The resistance rates to ofloxacin, norfloxacin, gentamicin, amikacin and streptomycin of the isolates from different types of water bodies were significantly different (P < 0.005). Plasmids of 92 isolates (56.8%) with the size of 0.90-158.83 kb and quantity of 1-6 were isolated, and 81 kinds of plasmid profiles were found. The isolates free of plasmids were resistant to two or more antibiotics. The isolates that had the same plasmid profiles were all different in resistance patterns. There was no relativity between the plasmid profiles and resistance pattern.
