Regional distribution of SGLT activity in rat brain in vivo Article

Yu, AS, Hirayama, BA, Timbol, G et al. (2013). Regional distribution of SGLT activity in rat brain in vivo . American Journal of Physiology - Cell Physiology, 304(3), C240-C247. 10.1152/ajpcell.00317.2012

cited authors

  • Yu, AS; Hirayama, BA; Timbol, G; Liu, J; Diez-Sampedro, A; Kepe, V; Satyamurthy, N; Huang, SC; Wright, EM; Barrio, JR

abstract

  • Na+-glu-cose cotransporter (SGLT) mRNAs have been detected in many organs of the body, but, apart from kidney and intestine, transporter expression, localization, and functional activity, as well as physiological significance, remain elusive. Using a SGLT-specific molecular imaging probe, a-methyl-4-deoxy-4-[18F]fluoro-D-glu-copyranoside (Me-4-FDG) with ex vivo autoradiography and im-munohistochemistry, we mapped in vivo the regional distribution of functional SGLTs in rat brain. Since Me-4-FDG is not a substrate for GLUT1 at the blood-brain barrier (BBB), in vivo delivery of the probe into the brain was achieved after opening of the BBB by an established procedure, osmotic shock. Ex vivo autoradiography showed that Me-4-FDG accumulated in regions of the cerebellum, hippocampus, frontal cortex, caudate nucleus, putamen, amygdala, parietal cortex, and paraventricular nucleus of the hypothalamus. Little or no Me-4-FDG accumulated in the brain stem. The regional accumulation of Me-4-FDG overlapped the distribution of SGLT1 protein detected by immunohistochemistry. In summary, after the BBB is opened, the specific substrate for SGLTs, Me-4-FDG, enters the brain and accumulates in selected regions shown to express SGLT1 protein. This localization and the sensitivity of these neurons to anoxia prompt the speculation that SGLTs may play an essential role in glucose utilization under stress such as ischemia. The expression of SGLTs in the brain raises questions about the potential effects of SGLT inhibitors under development for the treatment of diabetes. © 2013 the American Physiological Society.

authors

publication date

  • February 1, 2013

published in

Digital Object Identifier (DOI)

start page

  • C240

end page

  • C247

volume

  • 304

issue

  • 3