The nature of multiphoton fluorescence from red blood cells Conference

Saytashev, I, Murphy, M, Osseiran, S et al. (2016). The nature of multiphoton fluorescence from red blood cells . 9712 10.1117/12.2213422

cited authors

  • Saytashev, I; Murphy, M; Osseiran, S; Spence, DM; Evans, CL; Dantus, M

abstract

  • We report on the nature of multiphoton excited fluorescence observed from human erythrocytes (red blood cells RBC's) and their "ghosts" following 800nm sub-15 fs excitation. The detected optical signal is assigned as two-photon excited fluorescence from hemoglobin. Our findings are supported by wavelength-resolved fluorescence lifetime decay measurements using time-correlated single photon counting system from RBC's, their ghosts as well as in vitro samples of various fluorophores including riboflavin, NADH, NAD(P)H, hemoglobin. We find that low-energy and short-duration pulses allow two-photon imaging of RBC's, but longer more intense pulses lead to their destruction.

publication date

  • January 1, 2016

Digital Object Identifier (DOI)

volume

  • 9712