Multiphoton excited hemoglobin fluorescence and third harmonic generation for noninvasive microscopy of stored blood Article

Saytashev, I, Glenn, R, Murashova, GA et al. (2016). Multiphoton excited hemoglobin fluorescence and third harmonic generation for noninvasive microscopy of stored blood . 7(9), 3449-3460. 10.1364/BOE.7.003449

cited authors

  • Saytashev, I; Glenn, R; Murashova, GA; Osseiran, S; Spence, D; Evans, CL; Dantusw, M

abstract

  • Red blood cells (RBC) in two-photon excited fluorescence (TPEF) microscopy usually appear as dark disks because of their low fluorescent signal. Here we use 15fs 800nm pulses for TPEF, 45fs 1060nm pulses for three-photon excited fluorescence, and third harmonic generation (THG) imaging. We find sufficient fluorescent signal that we attribute to hemoglobin fluorescence after comparing time and wavelength resolved spectra of other expected RBC endogenous fluorophores: NADH, FAD, biliverdin, and bilirubin. We find that both TPEF and THG microscopy can be used to examine erythrocyte morphology non-invasively without breaching a blood storage bag.

publication date

  • September 1, 2016

Digital Object Identifier (DOI)

start page

  • 3449

end page

  • 3460

volume

  • 7

issue

  • 9