Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma Article

Bindukumar, B, Kawinski, E, Cherrin, C et al. (2004). Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma . Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 813(1-2), 113-120. 10.1016/j.jchromb.2004.09.014

cited authors

  • Bindukumar, B; Kawinski, E; Cherrin, C; Gambino, LM; Nair, MPN; Schwartz, SA; Chadha, KC

abstract

  • The role of prostate-specific antigen (PSA) during the onset of prostate cancer and subsequent tumor growth and metastasis is not well understood. We have developed a simple two step procedure, based on principles of hydrophobic charge-induction chromatography and molecular size chromatography to provide pure free-PSA (f-PSA) preparation that is free from all other known PSA complexes as well as human kallikrein 2 (hK2). The overall recovery of f-PSA is 72%. The isolated f-PSA consists of three known isoforms that corresponds to pI of 6.2, 6.4 and 7.2. f-PSA is enzymatically active and its enzymatic activity can be effectively neutralized by a serine protease inhibitor. © 2004 Elsevier B.V. All rights reserved.

authors

publication date

  • December 25, 2004

published in

Digital Object Identifier (DOI)

start page

  • 113

end page

  • 120

volume

  • 813

issue

  • 1-2