The Effect of Total Parenteral Nutrition (TPN) on Zinc (Zn) Retention in the Tissues of Rats Article

Chisholm, MM, Enrione, EB. (1995). The Effect of Total Parenteral Nutrition (TPN) on Zinc (Zn) Retention in the Tissues of Rats . JOURNAL OF THE AMERICAN DIETETIC ASSOCIATION, 95(9 SUPPL.), 10.1016/S0002-8223(95)00402-5

cited authors

  • Chisholm, MM; Enrione, EB

authors

abstract

  • Zn is regularly added to TPN solutions, however, requirements are poorly understood. Twenty-four male Fischer-344 rats were equally and randomly assigned to (n=6): a control (CON), a baseline (BS), an orally fed (OF), and an intravenously fed (IV) group. After a 9-day acclimation period, each rat (except CON) had a catheter surgically placed into the superior vena cava. During a 4-day post-operative recovery period, the rats were allowed ad libitum access to an amino-acid diet (AAD) and deionized and distilled (DDI) water. On day 13, the BS rats were exsanguinated and eviscerated. CON and OF rats continued to have ad libitum access to the AAD. The IV group had a TPN solution infused. The AAD and the TPN solution were isocaloric and insonitrogenous and provided sufficient macronutrients and kilocalories for normal growth and development of rats. All rats continued ad libitum access to DDI water. After 15 days, organs and sera were collected and all tissues were analyzed for Zn concentration by atomic absorption spectrophotometry. Of the nine tissues analyzed, the Zn concentration in the liver, kidney, and lung of the IV group was found to be significantly higher (p 0.01, p 0.01, p 0.01 respectively) from that of the CON, BS, and OF groups. Results indicated that the Zn concentration of liver, kidney, and lung increase with intravenously administered Zn in rats, and that serum Zn may not reflect tissue Zn status. This suggests that route of Zn intake affects tissue Zn concentration. © 1995 American Dietetic Association.

publication date

  • September 1, 1995

published in

Digital Object Identifier (DOI)

volume

  • 95

issue

  • 9 SUPPL.