Amplified fragment length polymorphism confirms reciprocal monophyly in Chrysomya putoria and Chrysomya chloropyga: A correction of reported shared mtDNA haplotypes Article

Picard, CJ, Villet, MH, Wells, JD. (2012). Amplified fragment length polymorphism confirms reciprocal monophyly in Chrysomya putoria and Chrysomya chloropyga: A correction of reported shared mtDNA haplotypes . 26(1), 116-119. 10.1111/j.1365-2915.2011.00976.x

cited authors

  • Picard, CJ; Villet, MH; Wells, JD

authors

abstract

  • Reinvestigation of mitochondrial haplotypes previously reported to be shared between the Afrotropical blowflies Chrysomya putoria Weidemann and Chrysomya chloropyga Weidemann (Diptera: Calliphoridae) revealed an error resulting from the misidentification of specimens. Preliminary amplified fragment length polymorphism (AFLP) analysis of the original and additional individuals again failed to find reciprocal monophyly, leading to a re-examination of the specimens for diagnostic male genitalic characters that were first described following the earlier study. Four of the original study specimens were found to have been misidentified, and definitive analysis of both mtDNA and AFLP genotypes using phylogenetic analysis and genetic assignment showed that each species was indeed reciprocally monophyletic. In addition to correcting the earlier error, this study illustrates how AFLP analysis can be used for efficient and effective specimen identification through both phylogenetic analysis and genetic assignment, and suggests that the latter method has special advantages for identification when no conspecific specimens are represented in the reference database. © 2011 The Authors. Medical and Veterinary Entomology © 2011 The Royal Entomological Society.

publication date

  • March 1, 2012

Digital Object Identifier (DOI)

start page

  • 116

end page

  • 119

volume

  • 26

issue

  • 1