Tubular cell HIV-entry through apoptosed CD4 T cells: A novel pathway Article

Singh, P, Goel, H, Husain, M et al. (2012). Tubular cell HIV-entry through apoptosed CD4 T cells: A novel pathway . VIROLOGY, 434(1), 68-77. 10.1016/j.virol.2012.09.009

cited authors

  • Singh, P; Goel, H; Husain, M; Lan, X; Mikulak, J; Malthotra, A; Teichberg, S; Schmidtmayerova, H; Singhal, PC

authors

abstract

  • We hypothesized that HIV-1 may enter tubular cells by phagocytosis of apoptotic fragments of HIV-1-infected T cells infiltrating tubular interstitium. The study was designed to evaluate the interaction of programmed death-1 (PD-1) receptors on CD4 T cells and programmed death ligand-1 (PD-L1) on tubular cells (HK2 and HRPTEC, primary tubular cells). Co-cultivation of HIV-1 infected lymphocytes (HIV-LY) with HK2s/HRPTECs resulted in T cell apoptosis, uptake of the apoptosed HIV-LY by HK2s/HRPTECs, tubular cell activation and HIV expression. Cytochalasin-B inhibited tubular cell HIV-LY uptake and anti-PD-L1 antibody inhibited HIV-LY apoptosis and tubular cell HIV-LY uptake, activation and HIV expression. These observations do indicate induction of apoptosis of T cells due to interaction of PD-1 and PD-L1 upon co-cultivation and subsequent phygocytosis of HIV-laden apoptotic bodies by tubular cells and thus the transfer of HIV-1 into tubular cells. These findings identify a novel pathway that facilitates HIV-1 entry into tubular cell. © 2012 Elsevier Inc.

publication date

  • December 5, 2012

published in

Digital Object Identifier (DOI)

start page

  • 68

end page

  • 77

volume

  • 434

issue

  • 1