Separation of DNA by capillary electrophoresis. Article

McCord, B, Hartzell-Baguley, B, King, S. (2008). Separation of DNA by capillary electrophoresis. . Methods in molecular biology (Clifton, N.J.), 384 415-429. 10.1007/978-1-59745-376-9_15

cited authors

  • McCord, B; Hartzell-Baguley, B; King, S

abstract

  • This chapter reports an overview of the analytical techniques used to perform genetic analysis of polymerase chain reaction products using capillary electrophoresis. Three separate but related techniques are described: the separation of native DNA with detection using fluorescent intercalating dyes, the separation of denatured DNA using fluorescently labeled primers, and the detection of single-strand conformation polymorphisms using denatured DNA separated under native conditions. The various techniques involve electrokinetic injection of the DNA onto a narrow band at the head of the column, sieving the DNA through various entangled polymer matrices, and detection via single or multichannel laser-induced fluorescence. Analytical protocols are provided, and a series of representative electropherograms are included.

authors

publication date

  • January 1, 2008

published in

Digital Object Identifier (DOI)

start page

  • 415

end page

  • 429

volume

  • 384