Determination of estradiol 2- and 4-hydroxylase activities by gas chromatography with electron-capture detection Article

Roy, D, Hachey, DL, Liehr, JG. (1991). Determination of estradiol 2- and 4-hydroxylase activities by gas chromatography with electron-capture detection . 567(2), 309-318. 10.1016/0378-4347(91)80137-2

cited authors

  • Roy, D; Hachey, DL; Liehr, JG

authors

abstract

  • A highly sensitive assay has been developed for measuring the rate of formation of 2-hydroxyestradiol and 4-hydroxyestradiol from estradiol by microsomal preparations. Catechol estrogens were converted to heptafluorobutyryl esters, which were separated by capillary column gas chromatography and quantified using electron-capture detection. 2-Hydroxyestradiol 17-acetate was used as an internal standard. The identity of catechol estrogen derivatives was verified by gas chromatography-mass spectrometry using negative-ion chemical ionization. Estrogens were identified by negative molecular ions and/or by characteristic fragments. This procedure permits quantification of catechol estrogens at the subpicogram level. The assay was validated by comparing estrogen 2- and 4-hydroxylase activities in microsomes from hamster and rat liver with values reported previously. © 1991.

publication date

  • July 5, 1991

Digital Object Identifier (DOI)

start page

  • 309

end page

  • 318

volume

  • 567

issue

  • 2