This work reports on the development of a flow through biosensor capable of detecting particles and cells using a novel 16 microelectrode array, using impedance techniques. The impedance spikes caused by particles/cells are used for detection. Particles/cells were classified based on the magnitude of impedance spikes and the slope of the bode plots obtained from impedance spectroscopy measurements. The live cells were found to have impedance spikes, about 10 percent higher than that of dead cells.