Electrochemical impedance based chiral analysis of anti-ascorbutic drug: L-Ascorbic acid and d-ascorbic acid using C-dots decorated conductive polymer nano-composite electrode Article

Pandey, I, Kant, R. (2016). Electrochemical impedance based chiral analysis of anti-ascorbutic drug: L-Ascorbic acid and d-ascorbic acid using C-dots decorated conductive polymer nano-composite electrode . Biosensors and Bioelectronics, 77 715-724. 10.1016/j.bios.2015.10.039

cited authors

  • Pandey, I; Kant, R

abstract

  • Clinical manifestations owing to l-ascorbic acid for scurvy as comparison to d-ascorbic acid and challenges of chiral purity are overcome by using chiral selective conductive polymer nanocomposite which mimics antibodies and enzymes. A novel chiral selective imprinted polyaniline-ferrocene-sulfonic acid film has been electrochemically fabricated on C-dots modified pencil graphite electrode. The performance of the obtained l-ascorbic acid or d-ascorbic acid chiral selective sensor was investigated by electrochemical impedance spectroscopy, cyclic and differential pulse voltammetry. The surface characteristics of the C-dots, chiral sensor before and after the de-doping of chiral d- and l-ascorbic acid were characterized by scanning electron microscopy, Raman spectroscopy and X-ray diffraction spectroscopy. Excellent recognition results were obtained by difference in electron transfer resistance. The proposed chiral sensor is capable of measuring d-ascorbic acid or l-ascorbic acid in aqueous as well as in real and commercial samples within the range of 0.020-0.187. nM and 0.003-0.232. nM with detection limit of 0.00073. nM and 0.00016. nM, respectively. The proposed method has also been examined for the chiral selective recognition of ascorbic acid isomers (d- and l-) quantitatively, in complicated matrices of real samples.

authors

publication date

  • March 15, 2016

published in

Digital Object Identifier (DOI)

start page

  • 715

end page

  • 724

volume

  • 77